[nanoPost] Label-free technique to measure biomolecular interactions in real-time

Company Spain

Surface Plasmon Resonance (SPR) is a powerful label-free technique to measure biomolecular interactions in real-time. There is a broad range of applications where the companies technology can be applied, wherever the study of biomolecular interactions is important: chemical and biochemical sensing, drug discovery, diagnostics, proteomics, genomics, forensics, food analysis, environmental monitoring…

Simple to use, the product provides the researcher with a lab tool to study the binding and dissociation of biomolecules. This is possible by the high resolution detection of changes in the refractive index on the surface of a sensor slide.

The product is a dual channel SPR instrument which integrates computer controlled pumps, valves and injection fluidics. Optics and upgradeable electronic modules are incorporated on the platform

 
System built-up

A SPR sensor based in the Kretschmann configuration is used in this instrument to achieve the resonant condition by total internal reflection. A polarised laser diode light strikes into two flow cells, with a volume of 300nl.  A prism and a multi-photodiode are located on concentric rotary stages with an angular resolution of 0.01º. β-SPR system has a detection limit of 10-5 in refractive index.

Replaceable sensor glass slides are 10 mm by 10mm coated with a 50nm Au layer. Self assembled monolayers or thin films add the surface chemistry functionality required for the sensor surface to detect a binding partner

Monitoring changes in refractive index as a function of time allows real-time analysis of the binding events occurring at the sensor surface due to the continuous flow of buffers and samples provided by the flow injection system.

APPLICATIONS

Several biomolecular interactions have been explored in SPR sensors as, for example, antibody-antigen, receptor-ligand or hormone-receptor because a wide variety of affinity ligands can be used: antibodies, antibody fragments, genetically engineered antibody derivatives, lectins, membrane receptors, nucleic acids, gene fusion protein, hormones, viruses, bacteria,…

Access the contact details for this post by subscribing to premium content membership. For more details on premium content membership see our membership and fees page >> or contact info@nanoposts.com, Tel: +44(0)1786 474768.